The award-winning museum's significant collection is displayed across several rooms and galleries, in a series of themed exhibits. Displays are regularly updated and temporary exhibitions are held from time to time. Visits for schools and other groups by appointment. Free audio guide available.

Port Macquarie was one of the earliest convict settlements outside of Sydney. The Museum building and additions are classified by the National Trust of Australia (NSW) as a rare example of a convict-built structure circa 1836. The historic Store building is State-significant.